unconjugate  (Bioss)

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Deficiency of Stomach-Type Claudin-18 in Mice Induces Gastric Tumor Formation Independent of H pylori Infection

doi: 10.1016/j.jcmgh.2019.03.003

Figure Lengend Snippet: Antibodies Used in Immunofluorescence Images and FACS Analysis

Article Snippet: TFF2 , Bioss Antibodies , bs-1921R , Unconjugate.

Techniques: Immunofluorescence

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Deficiency of Stomach-Type Claudin-18 in Mice Induces Gastric Tumor Formation Independent of H pylori Infection

doi: 10.1016/j.jcmgh.2019.03.003

Figure Lengend Snippet: Induction of spasmolytic polypeptide–expressing metaplasia (SPEM) in young and aged stCldn18–/– mice. ( A ) Immunofluorescence micrographs for TFF2 ( green ) and Pepsin C ( blue ) as SPEM cell markers co-stained with Ki-67 ( red ) and DAPI ( white ) in the stomach from stCldn18+/+ and stCldn18–/– mice (around 50 w.o.). Right panels are magnified images of the boxed regions in the left panels. SPEM cells were clearly observed in the stomach of the stCldn18–/– mice. The Ki-67 signals partially coincided with the SPEM cells ( arrows ). Representative images from at least 2 independent experiments are shown. Scale bars = 100 μm. ( B ) Expression levels of the tumor-related alternatively spliced variants of CD44, and GAPDH in the stomach from stCldn18+/+ and stCldn18–/– mice (8, 40, 60, and 100 w.o.) quantified by conventional RT-PCR. ( C ) Immunofluorescence micrographs for TFF2 ( green ) and Pepsin C ( blue ) as SPEM cell markers co-stained with CD44 ( red ) and DAPI ( white ) in the stomach from stCldn18+/+ and stCldn18–/– mice (around 50 w.o.). Right panels are magnified images of the boxed regions in the left panels. CD44-positive cells were clearly observed in the stomach of the stCldn18–/– mice. The CD44 signals partially coincided with the SPEM cells ( arrows ). Representative images from at least 3 independent experiments are shown. Scale bars = 100 μm.

Article Snippet: TFF2 , Bioss Antibodies , bs-1921R , Unconjugate.

Techniques: Expressing, Immunofluorescence, Staining, Reverse Transcription Polymerase Chain Reaction

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Deficiency of Stomach-Type Claudin-18 in Mice Induces Gastric Tumor Formation Independent of H pylori Infection

doi: 10.1016/j.jcmgh.2019.03.003

Figure Lengend Snippet: Development of intestinal metaplasia and ectopic gastric gland in aged stCldn18–/– mice. ( A ) Expression levels of Cldns in the stomach from s tCldn18+/+ (n = 6), and stCldn18–/– (n = 7), mice at 100 w.o. quantified by qRT-PCR. The expression levels of Cldn2 , 4 , 7 , and luCldn18 were significantly increased in the stomach of stCldn18–/– mice at old age. Gene expressions were normalized to GAPDH. Results are expressed as mean ± SD. Comparisons between 2 groups were performed using Student’s t test, and differences with P < .05 were considered statistically significant. n.s., not significant. * P < .05. ( B ) Immunofluorescence micrographs for Cldn2 or Cldn7 ( green ) co-stained with E-cadherin ( red ) and with or without villin ( blue ) in the stomach from stCldn18+/+ and stCldn18–/– mice (around 50 w.o.). Representative images from at least 3 independent experiments are shown. Scale bars = 100 μm. ( C ) Hematoxylin and eosin–stained images of the ectopic gastric gland in aged stCldn18–/– mice. Ectopic gastric glands were found in 1 of 3 old stCldn18–/– mice by examining stomach tissue slices. Right panels are magnified images of the boxed regions in the left panels. Representative images from at least 2 independent experiments are shown. Scale bars = 100, 50, 10 μm, left to right panel. ( D ) ( Left ) Immunofluorescence micrographs for TFF2 ( green ) and Pepsin C ( blue ) as SPEM cell markers co-stained with CD44 (red) and DAPI ( white ) in the ectopic gastric gland from stCldn18–/– mice (around 50 w.o.). (Right) Representative immunofluorescence micrographs for TFF2 ( green ), and Pepsin C ( blue ) as SPEM cell markers co-stained with Ki-67 ( red ) and DAPI ( white ) in the ectopic gastric gland from stCldn18–/– mice (around 50 w.o.). Right panels are magnified images of the boxed regions in the left panels. Representative images from at least 2 independent experiments are shown. Scale bars = 100, 50 μm, left to right panel. ( E ) ( Left ) Immunofluorescence micrographs for Cldn2 ( green ) co-stained for E-cadherin ( red ) and villin ( blue ) in the ectopic gastric gland from stCldn18–/– mice (around 50 w.o.). Right panels are magnified images of the boxed regions in the left panels. Representative images from at least 2 independent experiments are shown. Scale bars = 100, 50 μm, left to right panel. ( Middle, Right ) Immunofluorescence micrographs for Cldn4 or Cldn7 ( green ) co-stained for E-cadherin ( red ) in the ectopic gastric gland from stCldn18–/– mice (around 50 w.o.). Right panels are magnified images of the boxed regions in the left panels. Representative images from at least 2 independent experiments are shown. Scale bars = 100, 50 μm, left to right panel.

Article Snippet: TFF2 , Bioss Antibodies , bs-1921R , Unconjugate.

Techniques: Expressing, Quantitative RT-PCR, Immunofluorescence, Staining

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Deficiency of Stomach-Type Claudin-18 in Mice Induces Gastric Tumor Formation Independent of H pylori Infection

doi: 10.1016/j.jcmgh.2019.03.003

Figure Lengend Snippet: Antibodies Used in Immunofluorescence Images and FACS Analysis

Article Snippet: TFF2 , Bioss Antibodies , bs-1921R , Unconjugate.

Techniques: Immunofluorescence

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Deficiency of Stomach-Type Claudin-18 in Mice Induces Gastric Tumor Formation Independent of H pylori Infection

doi: 10.1016/j.jcmgh.2019.03.003

Figure Lengend Snippet: Antibodies Used in Immunofluorescence Images and FACS Analysis

Article Snippet: TFF2 , Bioss Antibodies , bs-1921R , Unconjugate.

Techniques: Immunofluorescence

Journal: Bioscience Reports

Article Title: Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice

doi: 10.1042/BSR20170695

Figure Lengend Snippet: Primer sequences for qRT-PCR

Article Snippet: The diluted primary antibody was added: rabbit antimouse polyclonal antibody SIRT1 (bs-1921R, Beijing Bioss Biotech Co. Ltd., Beijing, China) (1:500), rabbit antimouse monoclonal antibody Bax (ab32503, Abcam Inc., Cambridge, MA, U.S.A.), rabbit antimouse polyclonal antibody Bcl-2 (bs-20351R, Beijing Bioss Biotech Co. Ltd., Beijing, China) (1:500), rabbit antimouse polyclonal antibody p53 (bs-8687R, Beijing Bioss Biotech Co. Ltd., Beijing, China) (1:500) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (ab9485, Abcam Inc., Cambridge, MA, U.S.A.) (1:500) for overnight incubation and a wash with PBS three times at room temperature, each time for 5 min. Rabbit antimouse polyclonal antibody IgG labeled by horseradish peroxidase (HRP) was added and the tissues were shaken and incubated for 1 h at 37°C, washed with PBS three times, with 5 min intervals.

Techniques:

Journal: Bioscience Reports

Article Title: Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice

doi: 10.1042/BSR20170695

Figure Lengend Snippet: ( A ) Comparison of SIRT1 protein expression of lens tissue in the normal and model groups by immunohistochemistry; ( B ) comparison of the positive expression rates of SIRT1 protein in the normal and model groups; *, P <0.05 compared with the normal group.

Article Snippet: The diluted primary antibody was added: rabbit antimouse polyclonal antibody SIRT1 (bs-1921R, Beijing Bioss Biotech Co. Ltd., Beijing, China) (1:500), rabbit antimouse monoclonal antibody Bax (ab32503, Abcam Inc., Cambridge, MA, U.S.A.), rabbit antimouse polyclonal antibody Bcl-2 (bs-20351R, Beijing Bioss Biotech Co. Ltd., Beijing, China) (1:500), rabbit antimouse polyclonal antibody p53 (bs-8687R, Beijing Bioss Biotech Co. Ltd., Beijing, China) (1:500) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (ab9485, Abcam Inc., Cambridge, MA, U.S.A.) (1:500) for overnight incubation and a wash with PBS three times at room temperature, each time for 5 min. Rabbit antimouse polyclonal antibody IgG labeled by horseradish peroxidase (HRP) was added and the tissues were shaken and incubated for 1 h at 37°C, washed with PBS three times, with 5 min intervals.

Techniques: Expressing, Immunohistochemistry

Journal: Bioscience Reports

Article Title: Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice

doi: 10.1042/BSR20170695

Figure Lengend Snippet: ( A ) miR-211 expression and mRNA and protein expressions of SIRT1, Bcl-2, Bax, and p53 in mice lens; ( B ) strip chart of SIRT1, Bcl-2, Bax, and p53 proteins; ( C ) expressions of SIRT1, Bcl-2, Bax, and p53 proteins in mice lens; *, P <0.05 compared with the normal group.

Article Snippet: The diluted primary antibody was added: rabbit antimouse polyclonal antibody SIRT1 (bs-1921R, Beijing Bioss Biotech Co. Ltd., Beijing, China) (1:500), rabbit antimouse monoclonal antibody Bax (ab32503, Abcam Inc., Cambridge, MA, U.S.A.), rabbit antimouse polyclonal antibody Bcl-2 (bs-20351R, Beijing Bioss Biotech Co. Ltd., Beijing, China) (1:500), rabbit antimouse polyclonal antibody p53 (bs-8687R, Beijing Bioss Biotech Co. Ltd., Beijing, China) (1:500) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (ab9485, Abcam Inc., Cambridge, MA, U.S.A.) (1:500) for overnight incubation and a wash with PBS three times at room temperature, each time for 5 min. Rabbit antimouse polyclonal antibody IgG labeled by horseradish peroxidase (HRP) was added and the tissues were shaken and incubated for 1 h at 37°C, washed with PBS three times, with 5 min intervals.

Techniques: Expressing, Stripping Membranes

Journal: Bioscience Reports

Article Title: Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice

doi: 10.1042/BSR20170695

Figure Lengend Snippet: ( A ) Sequence of 3′-UTR region of SIRT1 mRNA binding with miR-211 ; ( B ) luciferase activity detection; *, P <0.05 compared with the NC group.

Article Snippet: The diluted primary antibody was added: rabbit antimouse polyclonal antibody SIRT1 (bs-1921R, Beijing Bioss Biotech Co. Ltd., Beijing, China) (1:500), rabbit antimouse monoclonal antibody Bax (ab32503, Abcam Inc., Cambridge, MA, U.S.A.), rabbit antimouse polyclonal antibody Bcl-2 (bs-20351R, Beijing Bioss Biotech Co. Ltd., Beijing, China) (1:500), rabbit antimouse polyclonal antibody p53 (bs-8687R, Beijing Bioss Biotech Co. Ltd., Beijing, China) (1:500) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (ab9485, Abcam Inc., Cambridge, MA, U.S.A.) (1:500) for overnight incubation and a wash with PBS three times at room temperature, each time for 5 min. Rabbit antimouse polyclonal antibody IgG labeled by horseradish peroxidase (HRP) was added and the tissues were shaken and incubated for 1 h at 37°C, washed with PBS three times, with 5 min intervals.

Techniques: Sequencing, Binding Assay, Luciferase, Activity Assay